A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings
نویسندگان
چکیده
منابع مشابه
An evidence for a potassium channel in endoplasmic reticulum based on single channel recording in bilayer lipid membrane
Introduction Numerous studies have demonstrated the presence of potassium selective channels in membranes internal organelles. These channels are essential to a large variety of cellular processes including intracellular 2+ a signaling, protein recycling, charge neutralization and cell protection. In contrast to the sarcoplasmic reticulum + here potassium channels have been clearly ...
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متن کاملan evidence for a potassium channel in endoplasmic reticulum based on single channel recording in bilayer lipid membrane
introduction numerous studies have demonstrated the presence of potassium selective channels in membranes internal organelles. these channels are essential to a large variety of cellular processes including intracellular 2+ a signaling, protein recycling, charge neutralization and cell protection. in contrast to the sarcoplasmic reticulum + here potassium channels have been clearly identified, ...
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Making and holding an artificial lipid bilayer horizontally in an aqueous solution within the microscopic working distance of ~100 μm are essential for simultaneous single molecule imaging and single ion-channel electrical current recording. However, preparation of such a lipid bilayer without a solid support is technically challenging. In a typical supported lipid bilayer, the asymmetric local...
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Black lipid membranes (BLMs) are widely used for recording the activity of incorporated ion channel proteins. However, BLMs are inherently unstable structures that typically rupture within a few hours after formation. Here, stabilized BLMs were formed using the polymerizable lipid bis-dienoyl phosphatidylcholine (bis-DenPC) on glass pipettes of approximately 10 microm (I.D.). After polymerizati...
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ژورنال
عنوان ژورنال: Micromachines
سال: 2020
ISSN: 2072-666X
DOI: 10.3390/mi11121070